A comparison of tissue-based and recombinant protein–based assays for detecting PCA-Tr/DNER-IgG
نویسندگان
چکیده
Purkinje cell cytoplasmic antibody, type Tr (PCATr) is typically encountered in patients with Hodgkin lymphoma–related paraneoplastic cerebellar ataxia. Immunohistochemical techniques, including indirect immunofluorescence assays (IFAs), employing rodent brain as substrate, reveal a specific immunoglobulin G (IgG) binding pattern on cerebellar Purkinje cell cytoplasm and molecular layer dendritic spines. PCA-Tr characterization as delta/ notch-like epidermal growth factor–related receptor (DNER)–IgG has permitted antigen-specific assay development. With rare exception, the phenotype encountered is cerebellar ataxia among patients with Hodgkin lymphoma. Tr autoimmunity is occasionally reported in some patients with noncerebellar neurologic disorders, and in others with non-Hodgkin lymphoma. We report the performance of 2 DNER-IgG assays, with comparison to the Mayo Clinic Neuroimmunology Laboratory’s IFA, among patients with known PCA-Tr autoimmunity, PCA-Tr-negative patients with lymphoma-related paraneoplastic disorders, and healthy controls.
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Standardized test for anti-Tr/DNER in patients with paraneoplastic cerebellar degeneration
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